il 6 (Proteintech)
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Proteintech
il 6
Il 6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+6/IL-6+Antibody/pmc13644541-48-59-61
Average 96 stars, based on 1204 article reviews
Il 6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+6/IL-6+Antibody/pmc13644541-48-59-61
Average 96 stars, based on 1204 article reviews
il 6 - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Renal Tubular Epithelial Cells Senescence Induced by 1‐Aminopyrene Activates Fibroblast via BRD4 Driven Senescence‐Associated Secretory Phenotype Signaling"
Article Title: Renal Tubular Epithelial Cells Senescence Induced by 1‐Aminopyrene Activates Fibroblast via BRD4 Driven Senescence‐Associated Secretory Phenotype Signaling
Journal: Journal of Biochemical and Molecular Toxicology
doi: 10.1002/jbt.71124
Figure Legend Snippet: 1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, Col1a2, TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and COL1A1 (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Techniques Used: Activation Assay, Staining, Immunofluorescence, Analysis, Quantitative RT-PCR, Expressing, Derivative Assay, Two Tailed Test
Figure Legend Snippet: AhR signaling is activated in RTECs by 1‐AP but is insufficient for SASP‐mediated fibroblast activation. (A) Representative images and quantification of immunofluorescence analysis of AHR expression in RTECs following 1‐AP treatment ± AhR inhibitor (10 μM) after 48 h. Scale bars, 20 µm. (B) Heatmap for RT‐qPCR analysis of AhR target genes ( CYP1A1, CYP1A2 ) and senescence markers ( p16, p21, p53 ) following 1‐AP treatment ± AhR inhibitor after 48 h. (C) Heatmap for RT‐qPCR analysis of SASP genes ( IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, CXCL1 ) following 1‐AP treatment ± AhR inhibitor after 48 h. Conditioned media (CM) transfer from NRK‐52E/RTECs treated with 1‐AP ± AhR inhibitor to NRK‐52F fibroblasts after 48 h showing (D) Heatmap for RT‐qPCR analysis of fibroblast activation and extracellular matrix genes ( α‐SMA, POSTN, Col1a2, TIMP‐1, CTGF ), (E–G) Quantification of immunofluorescence analysis of α‐SMA, POSTN and COL1A1 in NRK52F fibroblasts. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Techniques Used: Activation Assay, Immunofluorescence, Analysis, Expressing, Quantitative RT-PCR, Two Tailed Test
Figure Legend Snippet: BRD4 recruitment in RTECs sustains SASP transcription and paracrine fibroblast activation. (A) Representative images and quantification of immunofluorescence analysis of BRD4 expression in RTECs following 1‐AP treatment ± JQ1 (0.5 μM) after 48 h. Scale bars, 20 µm. (B) Heatmap for RT‐qPCR analysis of senescence regulators ( p16, p21, p53 ) and SASP genes ( IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, CXCL1 ) in RTECs following 1‐AP treatment ± JQ1 after 48 h. Conditioned media (CM) transfer from NRK‐52E/RTECs treated with 1‐AP ± JQ1 to NRK‐52F fibroblasts after 48 h showing (C) Heatmap for RT‐qPCR analysis of fibroblast activation and extracellular matrix genes ( α‐SMA, POSTN, Col1a2, TIMP‐1, CTGF ), (D–F) Quantification of immunofluorescence analysis of α‐SMA, POSTN and COL1A1 in NRK52F fibroblasts. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Techniques Used: Activation Assay, Immunofluorescence, Analysis, Expressing, Quantitative RT-PCR, Two Tailed Test
Figure Legend Snippet: BRD4 inhibition suppresses SASP and senescence while promoting apoptosis in 1‐AP exposed RTECs. (A) Representative images showing intracellular ROS (DCFDA; scale bar, 5 µm), DNA damage (alkaline comet assay; % DNA in tail), γ‐H2AX immunofluorescence (scale bar, 20 µm), SA‐β‐gal staining (scale bar, 5 µm), IL‐6 and TGF‐β1 immunofluorescence (scale bar, 20 µm), TUNEL staining, and p21 immunofluorescence (scale bar, 5 µm) in RTECs treated with DMSO, 1 µM 1‐AP, 1 µM 1‐AP + AhR inhibitor (10 µM), or 1 µM 1‐AP + JQ1 (0.5 µM) for 48 h. (B) Quantification of DCFDA fluorescence; (C) % DNA in tail from the alkaline comet assay; (D) γ‐H2AX‐positive cells; (E) SA‐β‐gal‐positive senescent cells; (F–G, I) quantification of IL‐6, TGF‐β1, and p21 immunofluorescence, respectively; (H) quantification of TUNEL‐positive cells; and (J, K) ELISA‐based quantification of IL‐6 and TGF‐β1 in conditioned media collected from RTECs following treatment for 48 h. All treatments were compared with the vehicle control (DMSO). Data are presented as mean ± SD, n = 3. Statistical analysis was performed using a two‐tailed unpaired Student's t ‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI was used as a nuclear counterstain. RFI, relative fluorescence intensity.
Techniques Used: Inhibition, Alkaline Single Cell Gel Electrophoresis, Immunofluorescence, Staining, TUNEL Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Control, Analysis, Two Tailed Test
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